tracheal overexpression of il-1β, irak-1 and traf-6 mrna in obese-asthmatic male wistar rats
Authors
abstract
objective(s): human and animal studies have shown a close relationship between obesity and asthma severity. here, we examined the effects of diet-induced obesity (dio) on the expression levels of il-1β, irak-1 and traf-6 mrna in male wistar rats tracheal after sensitization with ovalbumin (ova). materials and methods:twenty male wistar rats divided to four groups, included, control group with normal diet (c+nd), ova-sensitized group with normal diet (s+nd), control group with high-fat diet (c+hfd), and ova-sensitized group with high-fat diet (s+hfd). all animals fed for 8 weeks with standard pelts or high-fat diet, and then were sensitized and challenged with ova or saline for another 4 weeks with designed regimens. at the end of study, trachea isolated and examined for expression levels of il-1β, irak-1 and traf-6 mrna with rt-pcr method. results:diet-induced obesity groups developed increased weight, obesity indexes and lipid profiles (p<0.05 to p<0.001). the expression levels of il-1β mrna in ova-sensitization groups (s+nd and s+hfd) showed a significantly increased when compared with control group. also in s+hfd group, expression level of traf-6 mrna was higher than other groups (p<0.001). irak-1 expression level was high in s+hfd compared with control group.il-1β and traf-6 mrna correlated positively with obesity indexes. conclusion:the results showed that dio causes overexpression of il-1β, irak-1 and traf-6 mrna in an experimental model of asthma. our results suggested that in obese-asthmatic conditions locally production and activation of pro-inflammatory agents can be increased. these findings showed that possible mechanism for obesity-asthma relationships.
similar resources
Tracheal overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in obese-asthmatic male Wistar rats
Objective(s): Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). Materials and Methods:Twenty male Wistar rats divided to four groups, included, control group with ...
full textTracheal overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in obese-asthmatic male Wistar rats
OBJECTIVES Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). MATERIALS AND METHODS Twenty male Wistar rats divided to four groups, included, control group with no...
full textlung altered expression of il-1β mrna and its signaling pathway molecules in obese-asthmatic male wistar rats
epidemiological and clinical studies indicate a close relationship between obesity and asthma. here, we determined the impact of diet-induced obesity on the expression levels of il-1β, irak-1 and traf-6 mrna as well as il-1β protein level and pathological changes in male wistar rat’s lung after sensitization with ovalbumin (ova). twenty male wistar rats divided into four groups, control with no...
full textNeuroprotective Effects of Berberine After Severe Traumatic Brain Injury in Male Rats: The Role of IL-1β and IL 10
Background and purpose: Traumatic brain injury (TBI) is the leading cause of death in young people. Berberine is a flavonoid rich in barberries and many traditional Iranian herbal remedies that could be used in treatment of neurodegenerative diseases. These properties make it a viable treatment for neurodegenerative diseases. Therefore, this study intended to investigate the neuroprotective ac...
full textSimultaneous Effects of Aerobic Training and Berberine Chloride on Plasma Glucose, IL-6 and TNF-α in Type 1 Diabetic Male Wistar Rats
Background and Objectives: Aerobic training and berberine chloride include antioxidant and anti-inflammatory characteristics. In the current study, simultaneous effects of aerobic training and berberine chloride on plasma glucose, IL-6 and TNF-α were investigated in type 1 diabetic male Wistar rats. Materials and Methods: In this experimental study, 64 male Wistar rats were randomly divided in...
full textthe effect of aqueous extract of garlic on formalin-induced pain in male rats
چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...
15 صفحه اولMy Resources
Save resource for easier access later
Journal title:
iranian journal of basic medical sciencesجلد ۱۹، شماره ۴، صفحات ۳۵۰-۳۵۷
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023